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src targeting sirna sc 5266  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology src targeting sirna sc 5266
    Src Targeting Sirna Sc 5266, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 1300 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+src/c-Src+Antibody/pmc13046926-253-2-8
    Average 97 stars, based on 1300 article reviews
    src targeting sirna sc 5266 - by Bioz Stars, 2026-11
    97/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: Protein Phosphatase 2A Subunit B55 Alpha is Required for Angiotensin Type 2 Receptor Elicited Natriuresis
    Article Snippet: .. Kidney sections were used for confocal immunofluorescence microscopy for determining the degree of B55α knockdown, AT 2 R and NHE-3 trafficking, c-Src signaling, and lysosomal associated membrane protein 1 (LAMP1), early endosome antigen 1 (EEA1), and small GTPase Rab7 co-localization with AT 2 R. Kidney sections were incubated with the following single or pairs of primary antibodies in 1% milk tris-buffered saline with 0.02% Tween-20 (TBST 2 ) overnight at 4°C: 1) PP2A B55 (Cell Signaling; cat. no. 2290, 1:100); 2) AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 3) NHE-3 (Millipore; cat. no. MAB3136; 1:2,000); 4) p Try416 -Src (Santa Cruz; cat. no. sc-12350; 1:100); 5) c-Src (Santa Cruz; cat. no. sc-18; 1:100); 6) AT 1 R (custom raised 21 and kindly provided by Dr. Tahir Hussain at the University of Houston; 1:100); 7) LAMP1 (Invitrogen; cat. no. MA1-164; 1:50) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 8) EEA1 (Invitrogen; cat. no. 14-9114-82; 1:500) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 9) Rab7 (Cell Signaling; cat. no. 95746; 1:100) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100). .. Kidney sections were used for confocal immunofluorescence microscopy for determining the degree of B55α knockdown, AT 2 R and NHE-3 trafficking, c-Src signaling, and lysosomal associated membrane protein 1 (LAMP1), early endosome antigen 1 (EEA1), and small GTPase Rab7 co-localization with AT 2 R. Kidney sections were incubated with the following single or pairs of primary antibodies in 1% milk tris-buffered saline with 0.02% Tween-20 (TBST 2 ) overnight at 4°C: 1) PP2A B55 (Cell Signaling; cat. no. 2290, 1:100); 2) AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 3) NHE-3 (Millipore; cat. no. MAB3136; 1:2,000); 4) p Try416 -Src (Santa Cruz; cat. no. sc-12350; 1:100); 5) c-Src (Santa Cruz; cat. no. sc-18; 1:100); 6) AT 1 R (custom raised 21 and kindly provided by Dr. Tahir Hussain at the University of Houston; 1:100); 7) LAMP1 (Invitrogen; cat. no. MA1-164; 1:50) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 8) EEA1 (Invitrogen; cat. no. 14-9114-82; 1:500) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 9) Rab7 (Cell Signaling; cat. no. 95746; 1:100) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100).

    Microscopy:

    Article Title: Protein Phosphatase 2A Subunit B55 Alpha is Required for Angiotensin Type 2 Receptor Elicited Natriuresis
    Article Snippet: .. Kidney sections were used for confocal immunofluorescence microscopy for determining the degree of B55α knockdown, AT 2 R and NHE-3 trafficking, c-Src signaling, and lysosomal associated membrane protein 1 (LAMP1), early endosome antigen 1 (EEA1), and small GTPase Rab7 co-localization with AT 2 R. Kidney sections were incubated with the following single or pairs of primary antibodies in 1% milk tris-buffered saline with 0.02% Tween-20 (TBST 2 ) overnight at 4°C: 1) PP2A B55 (Cell Signaling; cat. no. 2290, 1:100); 2) AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 3) NHE-3 (Millipore; cat. no. MAB3136; 1:2,000); 4) p Try416 -Src (Santa Cruz; cat. no. sc-12350; 1:100); 5) c-Src (Santa Cruz; cat. no. sc-18; 1:100); 6) AT 1 R (custom raised 21 and kindly provided by Dr. Tahir Hussain at the University of Houston; 1:100); 7) LAMP1 (Invitrogen; cat. no. MA1-164; 1:50) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 8) EEA1 (Invitrogen; cat. no. 14-9114-82; 1:500) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 9) Rab7 (Cell Signaling; cat. no. 95746; 1:100) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100). .. Kidney sections were used for confocal immunofluorescence microscopy for determining the degree of B55α knockdown, AT 2 R and NHE-3 trafficking, c-Src signaling, and lysosomal associated membrane protein 1 (LAMP1), early endosome antigen 1 (EEA1), and small GTPase Rab7 co-localization with AT 2 R. Kidney sections were incubated with the following single or pairs of primary antibodies in 1% milk tris-buffered saline with 0.02% Tween-20 (TBST 2 ) overnight at 4°C: 1) PP2A B55 (Cell Signaling; cat. no. 2290, 1:100); 2) AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 3) NHE-3 (Millipore; cat. no. MAB3136; 1:2,000); 4) p Try416 -Src (Santa Cruz; cat. no. sc-12350; 1:100); 5) c-Src (Santa Cruz; cat. no. sc-18; 1:100); 6) AT 1 R (custom raised 21 and kindly provided by Dr. Tahir Hussain at the University of Houston; 1:100); 7) LAMP1 (Invitrogen; cat. no. MA1-164; 1:50) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 8) EEA1 (Invitrogen; cat. no. 14-9114-82; 1:500) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 9) Rab7 (Cell Signaling; cat. no. 95746; 1:100) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100).

    Knockdown:

    Article Title: Protein Phosphatase 2A Subunit B55 Alpha is Required for Angiotensin Type 2 Receptor Elicited Natriuresis
    Article Snippet: .. Kidney sections were used for confocal immunofluorescence microscopy for determining the degree of B55α knockdown, AT 2 R and NHE-3 trafficking, c-Src signaling, and lysosomal associated membrane protein 1 (LAMP1), early endosome antigen 1 (EEA1), and small GTPase Rab7 co-localization with AT 2 R. Kidney sections were incubated with the following single or pairs of primary antibodies in 1% milk tris-buffered saline with 0.02% Tween-20 (TBST 2 ) overnight at 4°C: 1) PP2A B55 (Cell Signaling; cat. no. 2290, 1:100); 2) AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 3) NHE-3 (Millipore; cat. no. MAB3136; 1:2,000); 4) p Try416 -Src (Santa Cruz; cat. no. sc-12350; 1:100); 5) c-Src (Santa Cruz; cat. no. sc-18; 1:100); 6) AT 1 R (custom raised 21 and kindly provided by Dr. Tahir Hussain at the University of Houston; 1:100); 7) LAMP1 (Invitrogen; cat. no. MA1-164; 1:50) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 8) EEA1 (Invitrogen; cat. no. 14-9114-82; 1:500) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 9) Rab7 (Cell Signaling; cat. no. 95746; 1:100) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100). .. Kidney sections were used for confocal immunofluorescence microscopy for determining the degree of B55α knockdown, AT 2 R and NHE-3 trafficking, c-Src signaling, and lysosomal associated membrane protein 1 (LAMP1), early endosome antigen 1 (EEA1), and small GTPase Rab7 co-localization with AT 2 R. Kidney sections were incubated with the following single or pairs of primary antibodies in 1% milk tris-buffered saline with 0.02% Tween-20 (TBST 2 ) overnight at 4°C: 1) PP2A B55 (Cell Signaling; cat. no. 2290, 1:100); 2) AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 3) NHE-3 (Millipore; cat. no. MAB3136; 1:2,000); 4) p Try416 -Src (Santa Cruz; cat. no. sc-12350; 1:100); 5) c-Src (Santa Cruz; cat. no. sc-18; 1:100); 6) AT 1 R (custom raised 21 and kindly provided by Dr. Tahir Hussain at the University of Houston; 1:100); 7) LAMP1 (Invitrogen; cat. no. MA1-164; 1:50) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 8) EEA1 (Invitrogen; cat. no. 14-9114-82; 1:500) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 9) Rab7 (Cell Signaling; cat. no. 95746; 1:100) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100).

    Membrane:

    Article Title: Protein Phosphatase 2A Subunit B55 Alpha is Required for Angiotensin Type 2 Receptor Elicited Natriuresis
    Article Snippet: .. Kidney sections were used for confocal immunofluorescence microscopy for determining the degree of B55α knockdown, AT 2 R and NHE-3 trafficking, c-Src signaling, and lysosomal associated membrane protein 1 (LAMP1), early endosome antigen 1 (EEA1), and small GTPase Rab7 co-localization with AT 2 R. Kidney sections were incubated with the following single or pairs of primary antibodies in 1% milk tris-buffered saline with 0.02% Tween-20 (TBST 2 ) overnight at 4°C: 1) PP2A B55 (Cell Signaling; cat. no. 2290, 1:100); 2) AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 3) NHE-3 (Millipore; cat. no. MAB3136; 1:2,000); 4) p Try416 -Src (Santa Cruz; cat. no. sc-12350; 1:100); 5) c-Src (Santa Cruz; cat. no. sc-18; 1:100); 6) AT 1 R (custom raised 21 and kindly provided by Dr. Tahir Hussain at the University of Houston; 1:100); 7) LAMP1 (Invitrogen; cat. no. MA1-164; 1:50) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 8) EEA1 (Invitrogen; cat. no. 14-9114-82; 1:500) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 9) Rab7 (Cell Signaling; cat. no. 95746; 1:100) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100). .. Kidney sections were used for confocal immunofluorescence microscopy for determining the degree of B55α knockdown, AT 2 R and NHE-3 trafficking, c-Src signaling, and lysosomal associated membrane protein 1 (LAMP1), early endosome antigen 1 (EEA1), and small GTPase Rab7 co-localization with AT 2 R. Kidney sections were incubated with the following single or pairs of primary antibodies in 1% milk tris-buffered saline with 0.02% Tween-20 (TBST 2 ) overnight at 4°C: 1) PP2A B55 (Cell Signaling; cat. no. 2290, 1:100); 2) AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 3) NHE-3 (Millipore; cat. no. MAB3136; 1:2,000); 4) p Try416 -Src (Santa Cruz; cat. no. sc-12350; 1:100); 5) c-Src (Santa Cruz; cat. no. sc-18; 1:100); 6) AT 1 R (custom raised 21 and kindly provided by Dr. Tahir Hussain at the University of Houston; 1:100); 7) LAMP1 (Invitrogen; cat. no. MA1-164; 1:50) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 8) EEA1 (Invitrogen; cat. no. 14-9114-82; 1:500) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 9) Rab7 (Cell Signaling; cat. no. 95746; 1:100) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100).

    Incubation:

    Article Title: Protein Phosphatase 2A Subunit B55 Alpha is Required for Angiotensin Type 2 Receptor Elicited Natriuresis
    Article Snippet: .. Kidney sections were used for confocal immunofluorescence microscopy for determining the degree of B55α knockdown, AT 2 R and NHE-3 trafficking, c-Src signaling, and lysosomal associated membrane protein 1 (LAMP1), early endosome antigen 1 (EEA1), and small GTPase Rab7 co-localization with AT 2 R. Kidney sections were incubated with the following single or pairs of primary antibodies in 1% milk tris-buffered saline with 0.02% Tween-20 (TBST 2 ) overnight at 4°C: 1) PP2A B55 (Cell Signaling; cat. no. 2290, 1:100); 2) AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 3) NHE-3 (Millipore; cat. no. MAB3136; 1:2,000); 4) p Try416 -Src (Santa Cruz; cat. no. sc-12350; 1:100); 5) c-Src (Santa Cruz; cat. no. sc-18; 1:100); 6) AT 1 R (custom raised 21 and kindly provided by Dr. Tahir Hussain at the University of Houston; 1:100); 7) LAMP1 (Invitrogen; cat. no. MA1-164; 1:50) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 8) EEA1 (Invitrogen; cat. no. 14-9114-82; 1:500) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 9) Rab7 (Cell Signaling; cat. no. 95746; 1:100) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100). .. Kidney sections were used for confocal immunofluorescence microscopy for determining the degree of B55α knockdown, AT 2 R and NHE-3 trafficking, c-Src signaling, and lysosomal associated membrane protein 1 (LAMP1), early endosome antigen 1 (EEA1), and small GTPase Rab7 co-localization with AT 2 R. Kidney sections were incubated with the following single or pairs of primary antibodies in 1% milk tris-buffered saline with 0.02% Tween-20 (TBST 2 ) overnight at 4°C: 1) PP2A B55 (Cell Signaling; cat. no. 2290, 1:100); 2) AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 3) NHE-3 (Millipore; cat. no. MAB3136; 1:2,000); 4) p Try416 -Src (Santa Cruz; cat. no. sc-12350; 1:100); 5) c-Src (Santa Cruz; cat. no. sc-18; 1:100); 6) AT 1 R (custom raised 21 and kindly provided by Dr. Tahir Hussain at the University of Houston; 1:100); 7) LAMP1 (Invitrogen; cat. no. MA1-164; 1:50) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 8) EEA1 (Invitrogen; cat. no. 14-9114-82; 1:500) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 9) Rab7 (Cell Signaling; cat. no. 95746; 1:100) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100).

    Article Title: Noncanonical functions of UGT2B17 promote castration-resistant prostate cancer progression
    Article Snippet: Baked and deparaffinized TMA slides were first incubated in cell conditioning 1 (Ventana) solution for 64 minutes at 91°C for antigen retrieval. .. TMA cores were then incubated for 2 hours at room temperature in a solution of antibodies specific to targets diluted in 1× TBS: UGT2B17 (dilution: 1:50, custom made) and c-Src (dilution: 1:25, sc-8056, Santa Cruz Biotechnology). ..

    Article Title: Non-canonical functions of UGT2B17 promote castrate-resistant prostate cancer progression
    Article Snippet: Baked and deparaffinized TMA slides were first incubated in cell conditioning 590 1 (CC1, Ventana) solution for 64 min at 91°C for antigen retrieval. .. TMA cores were then incubated for 2 591 hours at room temperature in a solution of antibodies specific to targets diluted in 1 x TBS: UGT2B17 592 (dilution: 1:50, custom made) and c-Src (dilution: 1:25, sc-8056, Santa Cruz). ..

    Article Title: Protein Phosphatase 2A Subunit B55 Alpha is Required for Angiotensin Type 2 Receptor Elicited Natriuresis
    Article Snippet: .. Kidney sections were incubated with the following single or pairs of primary antibodies in 1% milk TBST overnight at 4°C: 1) PP2A B55 (Cell Signaling; cat. no. 2290, 1:100); 2) AT R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 3) NHE-3 (Millipore; cat. no. MAB3136; 1:2,000); 4) p Try4 -Src (Santa Cruz; cat. no. sc-12350; 1:100); 5) c-Src (Santa Cruz; cat. no. sc-18; 1:100); 6) AT 1 R (custom raised and kindly provided by Dr. Tahir Hussain at the University of Houston; 1:100); 7) LAMP1 (Invitrogen; cat. no. MA1-164; 1:50) and AT R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 8) EEA1 (Invitrogen; cat. no. 14-9114-82; 1:500) and AT R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 9) Rab7 (Cell Signaling; cat. no. 95746;1:100) and AT R (Santa Cruz H-143; cat. no. sc-9040; 1:100). .. The secondary antibodies Alexa 647 conjugated donkey anti-rabbit (Invitrogen, cat. no. A31573; 1:500) or Alexa 488 conjugated donkey anti-mouse (Invitrogen; cat. nos.

    Saline:

    Article Title: Protein Phosphatase 2A Subunit B55 Alpha is Required for Angiotensin Type 2 Receptor Elicited Natriuresis
    Article Snippet: .. Kidney sections were used for confocal immunofluorescence microscopy for determining the degree of B55α knockdown, AT 2 R and NHE-3 trafficking, c-Src signaling, and lysosomal associated membrane protein 1 (LAMP1), early endosome antigen 1 (EEA1), and small GTPase Rab7 co-localization with AT 2 R. Kidney sections were incubated with the following single or pairs of primary antibodies in 1% milk tris-buffered saline with 0.02% Tween-20 (TBST 2 ) overnight at 4°C: 1) PP2A B55 (Cell Signaling; cat. no. 2290, 1:100); 2) AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 3) NHE-3 (Millipore; cat. no. MAB3136; 1:2,000); 4) p Try416 -Src (Santa Cruz; cat. no. sc-12350; 1:100); 5) c-Src (Santa Cruz; cat. no. sc-18; 1:100); 6) AT 1 R (custom raised 21 and kindly provided by Dr. Tahir Hussain at the University of Houston; 1:100); 7) LAMP1 (Invitrogen; cat. no. MA1-164; 1:50) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 8) EEA1 (Invitrogen; cat. no. 14-9114-82; 1:500) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 9) Rab7 (Cell Signaling; cat. no. 95746; 1:100) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100). .. Kidney sections were used for confocal immunofluorescence microscopy for determining the degree of B55α knockdown, AT 2 R and NHE-3 trafficking, c-Src signaling, and lysosomal associated membrane protein 1 (LAMP1), early endosome antigen 1 (EEA1), and small GTPase Rab7 co-localization with AT 2 R. Kidney sections were incubated with the following single or pairs of primary antibodies in 1% milk tris-buffered saline with 0.02% Tween-20 (TBST 2 ) overnight at 4°C: 1) PP2A B55 (Cell Signaling; cat. no. 2290, 1:100); 2) AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 3) NHE-3 (Millipore; cat. no. MAB3136; 1:2,000); 4) p Try416 -Src (Santa Cruz; cat. no. sc-12350; 1:100); 5) c-Src (Santa Cruz; cat. no. sc-18; 1:100); 6) AT 1 R (custom raised 21 and kindly provided by Dr. Tahir Hussain at the University of Houston; 1:100); 7) LAMP1 (Invitrogen; cat. no. MA1-164; 1:50) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 8) EEA1 (Invitrogen; cat. no. 14-9114-82; 1:500) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100); 9) Rab7 (Cell Signaling; cat. no. 95746; 1:100) and AT 2 R (Santa Cruz H-143; cat. no. sc-9040; 1:100).

    other:

    Article Title: Nucleus-translocated glucokinase functions as a protein kinase to phosphorylate TAZ and promote tumour growth
    Article Snippet: Normal mouse IgG (sc-2025), normal rabbit IgG (sc-2027), GST (sc-138), Importin α1 (sc-55538), c-SRC (sc-8056), CK2α (sc373894), PIN1 (sc-46660), TAZ (sc-518026), CTGF (sc-101586) and protein A/G agarose (sc-2003) were obtained from Santa Cruz Biotechnology.

    Article Title: Nucleus-translocated glucokinase functions as a protein kinase to phosphorylate TAZ and promote tumour growth.
    Article Snippet: Normal mouse IgG (sc-2025), normal rabbit IgG (sc-2027), GST (sc138), Importin α1 (sc-55538), c-SRC (sc-8056), CK2α (sc373894), PIN1 (sc-46660), TAZ (sc-518026), CTGF (sc-101586) and protein A/G agarose (sc-2003) were obtained from Santa Cruz Biotechnology.



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    fitc anti src antibody  (Santa Cruz Biotechnology)
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    Santa Cruz Biotechnology fitc anti src antibody
    Experimental validation. ( A , C , E , G , I and K ) Relative expression of <t>SRC,</t> <t>ESR1,</t> EGFR, PTGS2, PPARG and HSP90AA1 via RT-qPCR. ( B , D , F , H , J and L ) Typical pictures and scores of the relative expression of SRC, ESR1, EGFR, PTGS2, PPARG and HSP90AA1 via IHC; 20×, Scale bar: 100 μm; 40×, Scale bar: 50 μm. ** P < 0.01, and *** P < 0.001
    Fitc Anti Src Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cav1 tyrosine (Y)-14 phosphorylation induced by APC–PAR1 is mediated by c-Src. A – C, endothelial EA.hy926 cells were pretreated with dasatinib or DMSO prior to addition of APC. Cell lysates were then immunoblotted to detect Cav1 Y14 and c-Src Y416 phosphorylation as indicated. D – G, endothelial cells transfected with nonspecific (NS) or c-Src-specific siRNA were treated with or without APC, lysed, and immunoblotted as indicated. β-tubulin and GAPDH were used as loading controls. The data were quantified (mean ± SD) from four independent biological replicates and expressed as the fraction relative to the untreated control and analyzed by two-way ANOVA followed by Šídák's multiple comparisons test. ( B ) ∗ p = 0.0136; ( C ) ∗∗ p = 0.0038; ( F ) ∗∗∗ p = 0.0003; and ( G ) ∗ p = 0.0125. Student's unpaired t test, ( E ) ∗∗∗ p = 0.0002. APC, activated protein C; Cav1, caveolin-1; DMSO, dimethyl sulfoxide; PAR1, protease-activated receptor 1.

    Journal: The Journal of Biological Chemistry

    Article Title: Activated protein C drives β-arrestin-2- and c-Src-dependent phosphorylation of Cav1 and modulates Cav1 association with PAR1 and GRK5

    doi: 10.1016/j.jbc.2026.111190

    Figure Lengend Snippet: Cav1 tyrosine (Y)-14 phosphorylation induced by APC–PAR1 is mediated by c-Src. A – C, endothelial EA.hy926 cells were pretreated with dasatinib or DMSO prior to addition of APC. Cell lysates were then immunoblotted to detect Cav1 Y14 and c-Src Y416 phosphorylation as indicated. D – G, endothelial cells transfected with nonspecific (NS) or c-Src-specific siRNA were treated with or without APC, lysed, and immunoblotted as indicated. β-tubulin and GAPDH were used as loading controls. The data were quantified (mean ± SD) from four independent biological replicates and expressed as the fraction relative to the untreated control and analyzed by two-way ANOVA followed by Šídák's multiple comparisons test. ( B ) ∗ p = 0.0136; ( C ) ∗∗ p = 0.0038; ( F ) ∗∗∗ p = 0.0003; and ( G ) ∗ p = 0.0125. Student's unpaired t test, ( E ) ∗∗∗ p = 0.0002. APC, activated protein C; Cav1, caveolin-1; DMSO, dimethyl sulfoxide; PAR1, protease-activated receptor 1.

    Article Snippet: In this study, the following antibodies were used: mouse anti–PAR1 WEDE (Beckman Coulter, #IM2584), anti-Cav1 (CST, #3267S and BD, #610060), anti-Cav1 Y14 phospho antibody (CST, #3251), anti-βarr2 (Abcam, #ab54790), GAPDH (GeneTex, #GT239), c-Src Y416 (CST, #2101), anti-c-Src (CST, #2109), GRK5 (Santa Cruz, #sc-518005), GRK5 polyclonal antibody (Invitrogen, #PA5-96262) anti-GRK4-6 (Millipore, #05-466), anti-HA (CST, #3724S), anti-rabbit IgG (CST, #2729), anti-β-Tubulin (CST, #86298), anti–early endosome antigen-1 (BD Biosciences, #610457), anti-Vinculin (Sigma, #V9131), and anti-HA conjugated to horseradish peroxidase (Roche, #11667475001).

    Techniques: Phospho-proteomics, Transfection, Control

    βarr2 is required for APC-stimulated c-Src tyrosine (Y)-416 phosphorylation and Cav1 Y14 phosphorylation. Endothelial EA.hy926 cells were transfected with nonspecific (NS) or βarr2-specific siRNA, treated with APC, and c-Src Y416 phosphorylation ( A and B ) and Cav1 Y14 phosphorylation ( C and D ) were detected by immunoblotting as indicated. GAPDH was used as a loading control. The data were quantified (mean ± SD) from four independent biological replicates and expressed as the fraction relative to the untreated control and analyzed by two-way ANOVA followed by Šídák's multiple comparisons test. B, NS siRNA with and without APC, ∗∗ p = 0.0097; ns = not significant. D, NS siRNA with and without APC ∗∗∗ p = 0.0009; βarr2 siRNA with and without APC, ∗∗ p = 0.0061. APC, activated protein C; βarr2, β-arrestin-2; Cav1, caveolin-1.

    Journal: The Journal of Biological Chemistry

    Article Title: Activated protein C drives β-arrestin-2- and c-Src-dependent phosphorylation of Cav1 and modulates Cav1 association with PAR1 and GRK5

    doi: 10.1016/j.jbc.2026.111190

    Figure Lengend Snippet: βarr2 is required for APC-stimulated c-Src tyrosine (Y)-416 phosphorylation and Cav1 Y14 phosphorylation. Endothelial EA.hy926 cells were transfected with nonspecific (NS) or βarr2-specific siRNA, treated with APC, and c-Src Y416 phosphorylation ( A and B ) and Cav1 Y14 phosphorylation ( C and D ) were detected by immunoblotting as indicated. GAPDH was used as a loading control. The data were quantified (mean ± SD) from four independent biological replicates and expressed as the fraction relative to the untreated control and analyzed by two-way ANOVA followed by Šídák's multiple comparisons test. B, NS siRNA with and without APC, ∗∗ p = 0.0097; ns = not significant. D, NS siRNA with and without APC ∗∗∗ p = 0.0009; βarr2 siRNA with and without APC, ∗∗ p = 0.0061. APC, activated protein C; βarr2, β-arrestin-2; Cav1, caveolin-1.

    Article Snippet: In this study, the following antibodies were used: mouse anti–PAR1 WEDE (Beckman Coulter, #IM2584), anti-Cav1 (CST, #3267S and BD, #610060), anti-Cav1 Y14 phospho antibody (CST, #3251), anti-βarr2 (Abcam, #ab54790), GAPDH (GeneTex, #GT239), c-Src Y416 (CST, #2101), anti-c-Src (CST, #2109), GRK5 (Santa Cruz, #sc-518005), GRK5 polyclonal antibody (Invitrogen, #PA5-96262) anti-GRK4-6 (Millipore, #05-466), anti-HA (CST, #3724S), anti-rabbit IgG (CST, #2729), anti-β-Tubulin (CST, #86298), anti–early endosome antigen-1 (BD Biosciences, #610457), anti-Vinculin (Sigma, #V9131), and anti-HA conjugated to horseradish peroxidase (Roche, #11667475001).

    Techniques: Phospho-proteomics, Transfection, Western Blot, Control

    Model of PAR1–GRK5–βarr2–c-Src regulation by Cav1. A substantial population of PAR1–Cav1 and GRK5–Cav1 complexes coexist at the plasma membrane under basal conditions. APC bound to EPCR cleaves and activates PAR1, resulting in GRK5-dependent phosphorylation of the receptor C-terminal tail within 30 min. This may occur during a time when GRK5 dissociates from Cav1. Next, APC-activated and phosphorylated PAR1 recruits βarr2, which promotes activation of c-Src Y416 phosphorylation and c-Src-dependent Cav1 Y14 phosphorylation, a process that occurs after 30 min and is sustained through 90 min. At 30 min, GRK5–Cav1 may reassociate, whereas activated PAR1 and Y14 phosphorylated Cav1 remain dissociated through the 90 min interval. APC, activated protein C; βarr2, βarr2; Cav1, caveolin-1; EPCR, endothelial protein C receptor; GRK5, GPCR kinase 5; PAR1, protease-activated receptor-1.

    Journal: The Journal of Biological Chemistry

    Article Title: Activated protein C drives β-arrestin-2- and c-Src-dependent phosphorylation of Cav1 and modulates Cav1 association with PAR1 and GRK5

    doi: 10.1016/j.jbc.2026.111190

    Figure Lengend Snippet: Model of PAR1–GRK5–βarr2–c-Src regulation by Cav1. A substantial population of PAR1–Cav1 and GRK5–Cav1 complexes coexist at the plasma membrane under basal conditions. APC bound to EPCR cleaves and activates PAR1, resulting in GRK5-dependent phosphorylation of the receptor C-terminal tail within 30 min. This may occur during a time when GRK5 dissociates from Cav1. Next, APC-activated and phosphorylated PAR1 recruits βarr2, which promotes activation of c-Src Y416 phosphorylation and c-Src-dependent Cav1 Y14 phosphorylation, a process that occurs after 30 min and is sustained through 90 min. At 30 min, GRK5–Cav1 may reassociate, whereas activated PAR1 and Y14 phosphorylated Cav1 remain dissociated through the 90 min interval. APC, activated protein C; βarr2, βarr2; Cav1, caveolin-1; EPCR, endothelial protein C receptor; GRK5, GPCR kinase 5; PAR1, protease-activated receptor-1.

    Article Snippet: In this study, the following antibodies were used: mouse anti–PAR1 WEDE (Beckman Coulter, #IM2584), anti-Cav1 (CST, #3267S and BD, #610060), anti-Cav1 Y14 phospho antibody (CST, #3251), anti-βarr2 (Abcam, #ab54790), GAPDH (GeneTex, #GT239), c-Src Y416 (CST, #2101), anti-c-Src (CST, #2109), GRK5 (Santa Cruz, #sc-518005), GRK5 polyclonal antibody (Invitrogen, #PA5-96262) anti-GRK4-6 (Millipore, #05-466), anti-HA (CST, #3724S), anti-rabbit IgG (CST, #2729), anti-β-Tubulin (CST, #86298), anti–early endosome antigen-1 (BD Biosciences, #610457), anti-Vinculin (Sigma, #V9131), and anti-HA conjugated to horseradish peroxidase (Roche, #11667475001).

    Techniques: Clinical Proteomics, Membrane, Phospho-proteomics, Activation Assay

    Experimental validation. ( A , C , E , G , I and K ) Relative expression of SRC, ESR1, EGFR, PTGS2, PPARG and HSP90AA1 via RT-qPCR. ( B , D , F , H , J and L ) Typical pictures and scores of the relative expression of SRC, ESR1, EGFR, PTGS2, PPARG and HSP90AA1 via IHC; 20×, Scale bar: 100 μm; 40×, Scale bar: 50 μm. ** P < 0.01, and *** P < 0.001

    Journal: BMC Pharmacology & Toxicology

    Article Title: Investigating the mechanisms by which bisphenol A affects osteoarthritis through a novel network toxicology framework and experimental validation

    doi: 10.1186/s40360-026-01108-0

    Figure Lengend Snippet: Experimental validation. ( A , C , E , G , I and K ) Relative expression of SRC, ESR1, EGFR, PTGS2, PPARG and HSP90AA1 via RT-qPCR. ( B , D , F , H , J and L ) Typical pictures and scores of the relative expression of SRC, ESR1, EGFR, PTGS2, PPARG and HSP90AA1 via IHC; 20×, Scale bar: 100 μm; 40×, Scale bar: 50 μm. ** P < 0.01, and *** P < 0.001

    Article Snippet: The sections were incubated overnight at 4 °C with the following primary antibodies (all from Proteintech, diluted in PBS): SRC (1:200, 60315-1-Ig), ESR1 (1:200, 21244-1-AP), EGFR (1:1000, 18986-1-AP), PTGS2 (1:1000, 66351-1-Ig), PPARG (1:400, 16643-1-AP), and HSP90AA1 (1:400000, 13171-1-AP).

    Techniques: Biomarker Discovery, Expressing, Quantitative RT-PCR